Induction of TNF-alpha production from human peripheral blood monocytes with beta-1,3-glucan oligomer prepared from laminarin with beta-1,3-glucanase from Bacillus clausii NM-1

We prepared a beta-1,3-glucan oligomer (DP> or = 4) from laminarin (DP: 25-30) derived from Laminaria digitata with beta-1,3-glucanase, and examined its effect on human peripheral blood monocytes. Conditioned medium prepared by incubating monocytes (MC-CM) with the beta-1,3-glucan oligomer showed strong inhibitory activity against the proliferation of human leukemic U937 cells. Since the beta-1,3-glucan oligomer had no direct cytotoxic effect on U937 cells up to 1000 microg/ml, the cytotoxicity of the MC-CM may be due to cytotoxic cytokines produced from monocytes stimulated by the beta-1,3-glucan oligomer. On the other hand, the MC-CM prepared with original laminarin had little effect on the growth of U937 cells. The cytotoxicity of the MC-CM prepared with the beta-1,3-glucan oligomer was significantly reduced by an anti-TNF-alpha antibody, but the anti-TNF-beta antibody had no effect. Our results suggest that the enzymatically depolymerized beta-1,3-glucan oligomer induces TNF-alpha production from human monocytes.     

Dietary CLA and DHA modify skin properties in mice

This study investigated the influence of PUFA on the properties of mouse skin. Mice (3 wk old) were given free access to oils high in linoleic acid, CLA, or DHA for 4 wk. At the end of the experiment, their skins were compared by both biochemical and histological methods. No significant differences in lipid and collagen contents were detected among treatments, although the FA composition in the skin was altered depending upon the FA composition of the supplemented oils. Electron microscopy revealed that the subcutaneous tissue layers in the CLA and DHA groups were significantly thinner than that in the high linoleic acid group, whereas no differences in the thickness of dermis layers were observed among the three groups. These results suggest that skin properties in mice are readily modified by dietary FA sources within 4 wk of dietary oil supplementation.     

A Two-Dimensional Bandwidth Extrapolation Technique for Polarimetric Synthetic Aperture Radar Images

The resolution of a synthetic aperture radar (SAR) image, in range and azimuth, is determined by the transmitted bandwidth and the synthetic aperture length, respectively. Various superresolution techniques for improving resolution have been proposed, and we have proposed an algorithm that we call polarimetric bandwidth extrapolation (PBWE). To apply PBWE to a radar image, one needs to first apply PBWE in the range direction and then in the azimuth direction, or vice versa . In this paper, PBWE is further extended to the 2-D case. This extended case (2D-PBWE) utilizes a 2-D polarimetric linear prediction model and expands the spatial frequency bandwidth in range and azimuth directions simultaneously. The performance of the 2D-PBWE is shown through a simulated radar image and a real polarimetric SAR image     

New DC Chopper Circuits Using Fast-Switching Reverse-Conducting Thyristors for Low-Voltage DC Motor Control

Fast- switching reverse-conducting thyristors have been developed and used in dc chopper apparatus to control the speed of highvoltage dc traction motors. The newly developed dc chopper circuit and its application for low-voltage dc motor control are discussed. The fast-switching reverse-conducting thyristor is utilized as the main thyristor to control load current.     

On the heat removal characteristics and the analytical model of a thermal energy storage capsule using gelled Glauber's salt as the PCM

An analytical and experimental investigation was performed on a heat removal process of the thermal energy storage (TES) capsule, using gelled Glauber's salt. Transient heat flux at the capsule wall was measured for various cooling conditions. In cases where the initial temperature was lower than the saturation temperature of the phase change material (PCM), numerical analyses were performed, approximating the process by a relaxation phenomenon where crystals grew around crystal nuclei. The results of the numerical solution agreed very well with the experimental results.     

Absorption spectra and tautomerism of aminonaphthazarins

2-n-Butylaminonaphthazarin (2) absorbs at 499 and 532 nm in ethanol but at 539 and 577 nm in alkali solution, while 2, 6-bis(n-butylamino)naphthazarin (3) absorbs at 461, 500 and 540 nm in ethanol but at 511 and 545 nm in alkali solution, with only a small bathochromic shift of 5 nm. The absorption curve of 3 was changed by addition of alkali and the λD_max at around 461 nm completely disappeared. In the case of 2-bromo-3, 6-bis(n-butylamino)naphthazarin (4), tautomerism between the 1, 4-quinone and 1, 5-quinone forms was observed and the isosbestic point was observed at 480 nm. Dye 2 easily deprotonated to give the 1, 4-dianion, but dyes 3 and 4 existed as tautomeric mixtures in solution and shifted to 1, 5-quinone forms in alkali solution.     

Surface/Thickness Translated: Design-Build as Vehicle

The Building-Design Workshop at the Taubman College of Architecture and Urban Planning pursues the integration of teaching, design research, and the design-build process. The program brings University-sponsored projects into the teaching context, offering a unique forum for testing pedagogies about making. Collaborating with faculty on both architectural and constructional aspects of a project, students are given a valuable occasion to realize a design in built form. The project shown here is a renovation of the College's Faculty Resource Room (FRR), a support facility for the research and teaching of the Architecture and Planning faculty.     

Entirely VPE-grown 1-5?m DFB lasers with low threshold currents

Entirely hydride VPE-grown 1.5?m DFB lasers have been obtained by means of high controllability in film thickness and alloy composition for the GalnAsP/InP system. A low threshold current of 13 mA was achieved by improving the growth method for the layer burying the grating. High uniformity in threshold current and lasing wavelength (Ith = 27.3 ± 9.7 mA, ? = 15571 ± 12?) was obtained.     

Analysis of chirp power penalty in 1.55-µm DFB-LD high-speed optical fiber transmission systems

In a 1.55-μm high-speed transmission system using a DFB-LD, the chirp effect in the LD is a factor limiting transmission span length. In order to evaluate the chirp effect, we derive expressions for the chirp power penalty for two cases: the cases of the chirp occurring in both edges of the pulse and in the whole time of the pulse. The calculations based on the results of the LD chirp measurement predict that the chirp effect will be significant above 2 Gbit/s even if the zero dispersion wavelength of fiber is shifted to the 1.55-μm band. Transmission experiments performed at 1.2 Gbit/s and 2.4 Gbit/s verify this prediction.     

Penetration and stainability of modified Sato's lead staining solution.

The penetration and stainability of modified Sato's lead staining solution containing calcined lead citrate were studied. Modified Sato's lead solution was preserved for 1 week and for 2 years, each at room temperature and at 4 degrees C. Specimens were stained with these solutions to measure the stainability. After 2-min staining, specimens were stained to the depth of 1.0-1.2 microns even when there had been 2-year preservation of the staining solution. This modified solution could be preserved for a long time and good penetration and stainability could still be obtained. This solution is also suitable for the observation of semithin sections.